|
KMID : 1161420180210111173
|
|
Journal of Medicinal Food
2018 Volume.21 No. 11 p.1173 ~ p.1187
|
|
|
Effects of Herbal Formulas Bojungikgi-tang and Palmijihwang-hwan on Inflammation in RAW 264.7 Cells and the Activities of Drug-Metabolizing Enzymes in Human Hepatic Microsomes
|
|
Jin Seong-Eun
Ha Hye-Kyung
Shin Hyeun-Kyoo
|
|
|
Abstract
|
|
|
|
In the present study, Bojungikgi-tang (BJIKT: Buzhongyiqi-tang, Hochuekki-to) and Palmijihwang-hwan (PMJHH: Baweidihuang-wan, Hachimijio-gan), traditional herbal formulas, investigated anti-inflammatory efficacies in murine macrophage cell line and the influence on the activities of drug-metabolizing enzymes (DMEs). The anti-inflammatory potentials of the herbal formulas were evaluated to inhibit the production of the inflammatory mediators and cytokines and the protein expression of inducible nitric oxide and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. The activities of the major human DMEs, cytochrome P450 (CYP450) and UDP-glucuronosyltransferase (UGT) isozymes, were measured by in vitro enzyme assay systems. BJIKT and PMJHH significantly suppressed the prostaglandin E2 (PGE2) production (IC50?=?317.3 and 282.2?μg/mL, respectively) and the protein expression of COX-2 in LPS-treated RAW264.7 cells. On the human microsomal DMEs, BJIKT inhibited the activities of CYP1A2 (IC50?=?535.05?μg/mL), CYP2B6 (IC50 > 1000?μg/mL), CYP2C9 (IC50?=?800.78?μg/mL), CYP2C19 (IC50?=?563.11?μg/mL), CYP2D6 (IC50 > 1000?μg/mL), CYP2E1 (IC50 > 1000?μg/mL), CYP3A4 (IC50?=?879.60?μg/mL), UGT1A1 (IC50 > 1000?μg/mL), and UGT1A4 (IC50 > 1000?μg/mL), but it showed no inhibition of the UGT2B7 activity at doses less than 1000?μg/mL. PMJHH inhibited the CYP2D6 activity (IC50?=?280.89?μg/mL), but IC50 values of PMJHH exceeded 1000?μg/mL on the activities of CYP1A2, CYP2C19, CYP2E1, and CYP3A4. At concentrations less than 1000?μg/mL, PMJHH did not affect the activities of CYP2B6, CYP2C9, UGT1A1, UGT1A4, and UGT2B7. The results indicate that both BJIKT and PMJHH may be potential candidates to prevent and treat PGE2- and COX-2-mediated inflammatory diseases. In addition, this study will expand current knowledge about herb?drug interactions by BJIKT and PMJHH.
|
|
|
KEYWORD
|
|
|
anti-inflammation, cytochrome P450 (CYP450), herbal formulas, herb?drug interactions, UDP-glucuronosyltransferase (UGT)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|